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Electron Microscopic Study on The Calcification of Tooth Cementum

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Abstract


Calcification of tooth cementum remains a controversial subject in spite of reports describing the positive sighting of matrix vesicles on cementum. The present study describes whether matrix vesicles are involved in the mineraization of
cementum,
if
so, whether they take an important part. The serial section of developing root of postunatal 15 day rats was observed under transmission electron microscope prepared with hydrous and anhydrous method. A few number of matrix
vesicles(0.54¡¾0.44/10§­)
were found at early stage of the formation of cellular cementum. Some of matrix vesicles were, however, remained uncalcified even within the mineralizing front. Any matrix vesicles were not spatially related to the calcifying spots and Ca-P
patients in
the matrix vesicle did not pierce the membrnane of matrix vesicle and didn't extrude into extrude into extracelular space. And Ca-P crystals in the matrix vesicle were not leaded to calcification of organic matrix. Thus, calcification within the
matrix
vesicles was the localized phenomina limiting to the vesicles.. By observing the serial sections, deposition of small mineral particles within the cross-sectioned collagen fibril bundles were found. This results suggest that cementum may be
calcified by
the calcification of collagenous fibrils.

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